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Coding

Part:BBa_K801060

Designed by: Lara Kuntz   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 17:40, 26 September 2012 by Simon Heinze (Talk | contribs)

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.

This part contains the (+)-Limonene Synthase 1 of Citrus limon. It is preceeded by the yeast consensus sequence for improved expression and carries a C-Terminal Strep-Tag for purification or detection by westernblot. It is an improved version of BBa_I742111. Also see the experience pages of BBa_I742111 and BBa_K118025.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal SapI.rc site found at 107


[edit]
Categories
//cds
//cds/biosynthesis
//chassis/eukaryote/yeast
Parameters
None