Coding
fhlA-E363

Part:BBa_K778002:Design

Designed by: Yuka Yamazaki   Group: iGEM12_UT-Tokyo   (2012-09-22)
Revision as of 17:09, 26 September 2012 by YutaOtsuka (Talk | contribs) (→‎Design Notes)

fhlA-E363G (engineered trascriptional activator fhlA with a mutation)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

E363G ( to , glutamic acid to glycine) point mutation was generated by inverce PCR. This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009(1)). However, in our (team UT-Tokyo 2012) research, this mutation is

Source

BBa_K778001

References