Composite

Part:BBa_K733010:Design

Designed by: WANG, Yuqi   Group: iGEM12_HKUST_Hong_Kong   (2012-09-16)
Revision as of 16:34, 26 September 2012 by Yliac (Talk | contribs) (→‎Source)

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Ptms+RBS+ydcD.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None

Source

We digest Ptms and RBS+ydcD and ligate them.

Backbone: Ptms in pSB1C3. Enzyme used: SpeI and PstI-HF.

Insert: RBS+ydcD. Enzyme used: XbaI and PstI-HF. Purified by gel purification.

References