Composite

Part:BBa_K733010

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 16:32, 26 September 2012 by Yliac (Talk | contribs)

Ptms+RBS+ydcD.

This construct contains a low efficient constitutive promoter pTms with an antitoxin gene ydcD (endB). The gene ydcD can express a protein named YdcD, which can counteract with a protein EndoA expressed by a toxin gene ydcE (ndoA). (Pellegrini et al., 2005) Besides, the low efficiency promoter will contribute to the low level expression of YdcD.

Ptms promoter: BBa_K733001

ydcD: BBa_K733003

ydcE: BBa_K733004

Cell Growth Inhibition Device: BBa_K733012


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Pellegrini O, Mathy N, Gogos A, Shapiro L, and Condon C. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor.." Molecular microbiology. 56.5 (2005): 1139-1148. Print.

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