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Coding
CysI

Part:BBa_K896001

Designed by: Shih-Hao Chen, Yen-Chen Lo,Ting-Yi Lin   Group: iGEM12_NYMU-Taipei   (2012-09-21)
Revision as of 16:29, 26 September 2012 by Lo6203 (Talk | contribs)

CysI (Sulfite reductase)

borderless

Our engingeered cyanobacteria can remove Sulfur Oxides (SOX, SO2),main precursors of air pollution and produce H2S for futher uses(Sulfide-Quinone Reductase,sqr).

S5.jpg



CysI( surfur reductase) :This is a enzyme that can reduce (HSO3)- to H2S using the embedded electron transfer system in pseudomonas aeruginosa PAOI.


[http://www.rcsb.org/pdb/explore/explore.do?structureId=3MM5 sulfite reductase in complex with the substrate sulfite]





Usage and Biology

1. From clinical research, we fund out that Pseudomonas aeruginosa can produce great amount H2S.So, we logical assumed there are some pivotal enzymes can transfer (HSO3)- to H2S.

2. We searched for [http://www.genome.jp/kegg/ KEGG] and [http://www.ncbi.nlm.nih.gov/ NCBI] and found out CysI, a sulfite reductase inside [http://www.ncbi.nlm.nih.gov/protein/NP_250529.1 Pseudomonas aeruginosa PAOI].

3.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 655
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 655
    Illegal PstI site found at 121
    Illegal NgoMIV site found at 394
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 722
    Illegal AgeI site found at 94
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 130
    Illegal BsaI site found at 1452

Functional Parameters

keggPseudomonas aeruginosa PAO1: PA1838
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Categories
Parameters
keggPseudomonas aeruginosa PAO1: PA1838