Coding
fhlA

Part:BBa_K778001:Design

Designed by: Tomohito Minakuchi   Group: iGEM12_UT-Tokyo   (2012-09-22)
Revision as of 16:18, 26 September 2012 by YutaOtsuka (Talk | contribs) (→‎Design Notes)

fhlA: a transcriptional activator for enhanced hydrogen production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original fhlA gene we cloned from E. coli JM109 has recognition sites of PatⅠ.This illegal (inappropriate for biobrick) site was deleted by overlap extension PCR generating mutation (G to A).


At 1906bp mutation (A to G, Thr to Ala) was found in this biobrick part(different from E. coli K-12 MG1655). can be

Source

Escherichia coli (JM109)

References