Regulatory

Part:BBa_K837000:Design

Designed by: Sun Xiaochen   Group: iGEM12_Tsinghua-D   (2012-09-26)
Revision as of 16:18, 26 September 2012 by Registry (Talk | contribs)

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RNAT.1-eGFP-pSB1C3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 790
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 791
    Illegal PstI site found at 805
    Illegal NotI site found at 7
    Illegal NotI site found at 798
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 791
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 791
    Illegal PstI site found at 805
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The SD sequence that benefits the bond between the RNA and the ribosome is designed in front of the eGFP sequence.


Source

The RNAT sequence is designed by us, using computational method. The eGFP gene is from the iGEM part,BBa_I714891.

References