Composite

Part:BBa_K887000:Design

Designed by: LIN I   Group: iGEM12_NCTU_Formosa   (2012-08-19)
Revision as of 16:17, 26 September 2012 by Pearl (Talk | contribs) (→‎Design Notes)

Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD+37℃ induced RBS+tetR+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6049
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6938
    Illegal AgeI site found at 3158
    Illegal AgeI site found at 4143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2744
    Illegal BsaI site found at 5835
    Illegal BsaI site found at 6092
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1430
    Illegal BsaI.rc site found at 3563


Design Notes

The first part of this complete biobrick is built in Plac, two strong and one weak expressing RBS, Alss, ilvC, ilvD, 37°C regulator RBS, tetR and double terminator. Before the enzymes,there are zic fingers from 2010 Slovenia iGEM team. And we also corrected the missing base pair of DNA program from 2010 Slovenia iGEM team.

Source

References