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Part:BBa_K897318:Design

Designed by: Muyuan Chen   Group: iGEM12_SEU_O_China   (2012-09-18)
Revision as of 15:35, 26 September 2012 by Arielinseu (Talk | contribs) (→‎References)

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Paired termini structure


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 59
    Illegal NotI site found at 84
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 59
    Illegal BamHI site found at 53
    Illegal XhoI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With T7


Source

no

References

Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli, Nobutaka Nakashima, Tomohiro Tamura and Liam Good, Nucleic Acids Research, 2006, Vol. 34, No. 20