Generator
Part:BBa_K738002:Design
Designed by: Huachun Liu Group: iGEM12_ZJU-China (2012-08-09)
Revision as of 15:09, 26 September 2012 by Smile (Talk | contribs) (New page: __NOTOC__ <partinfo>BBa_K738002 short</partinfo> <partinfo>BBa_K738002 SequenceAndFeatures</partinfo> ===Design Notes=== Generally, RNA strands have a half-life that ranges between 40 s...)
Theophyline riboswitch regulated RNA Scaffold (clover vision 2)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.
Source
Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).
References
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).