Coding

Part:BBa_K738004

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-08-09)
Revision as of 14:25, 26 September 2012 by Smile (Talk | contribs)

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FA-2X-MS2: Split GFP N-terminal domain fused with MS2 protein

FA is half of EGFP with 2X linker[Gly4Ser]2, fusion expressed with MS2, a phage coat domain.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 798
    Illegal EcoRI site found at 1182
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 835
    Illegal BsaI site found at 1219


[edit]
Categories
Parameters
n/aFA-2X-MS2: Half of split GFP