Composite

Part:BBa_J37034:Experience

Designed by: John Sy   Group: iGEM06_Imperial   (2006-08-23)
Revision as of 13:46, 26 September 2012 by Nongchaoer (Talk | contribs) (→‎User Reviews)

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This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J37034

This part was tested by Tsinghua 2012 team.

LuxI is a AHL synthase and GFP work as a reporter to show the expression of LuxI. This part can work as a signal sender.

User Reviews

UNIQc28a6c61af10b9a3-partinfo-00000000-QINU UNIQc28a6c61af10b9a3-partinfo-00000001-QINU This part was tested by Tsinghua 2012 team.

When we first use this part, it doesn't work at all. When we digest the plasmid by EcoR1 and Pst1, there are three bands appear and no band is correct. Then we sequence the part and we get a conclusion that the sequence of this part from iGEM 2011 kit is wrong.

We improve this part by ourselves. We change GFP to RFP protein and move RFP upstream of LuxI. This red fluorescent protein is much easier to detect even by your eyes and you don't have to use microscope.