Plasmid_Backbone
pPICZα

Part:BBa_K805020:Design

Designed by: Tang Yong   Group: iGEM12_HUST-China   (2012-09-20)
Revision as of 13:13, 26 September 2012 by HUST XueYu (Talk | contribs) (→‎Design Notes)

pPICZα


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1208
    Illegal PstI site found at 2447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1208
    Illegal PstI site found at 2447
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1208
    Illegal BglII site found at 1
    Illegal BamHI site found at 2087
    Illegal BamHI site found at 2861
    Illegal XhoI site found at 1184
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1208
    Illegal PstI site found at 2447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1208
    Illegal PstI site found at 2447
    Illegal NgoMIV site found at 1520
    Illegal NgoMIV site found at 1976
    Illegal NgoMIV site found at 3619
    Illegal NgoMIV site found at 3680
    Illegal AgeI site found at 2240
    Illegal AgeI site found at 2580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2237
    Illegal BsaI.rc site found at 2844
    Illegal BsaI.rc site found at 3043
    Illegal BsaI.rc site found at 4041


Design Notes

This plasmid is composed of BBa_K805011 and BBa K805020. sequence confirmed.

Source

yes

References