Protein_Domain
CBDclos
Part:BBa_K863111
Designed by: Moritz Müller Group: iGEM12_Bielefeld-Germany (2012-09-18)
Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
Cellulose binding Domain (CBDclos) of Clostridium cellulovorans [http://www.ncbi.nlm.nih.gov/nuccore/M73817 cellulose binding protein gene (cbp A)] in Standard 25 (Freiburg).
Usage and Biology
The CBDclos is an N-terminal domain quite close to the start of the protein-sequence (Figure 1). The NCBI-BLAST identified 92 amino acids (276 bases; green bar) as the protein domain. 12 bases (4 amino acids) were taken as conserved sequence upstream and 6 bases (2AS) downstream of the domain to secure natural folding of the domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 310 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//binding/cellulose
//cds
//proteindomain/binding
//cds
//proteindomain/binding
family | Cellulose Binding Modul family 3 (pfam00942/cl03026) |
function | cellulose binding |
origin | Clostridium cellulovorans cellulose binding protein gene (cbp A) |
strain | 743B |
swisspro | M73817 |
type | Cellulose binding domain |
uniprot | P38058 |