Coding
Part:BBa_K801066
Designed by: Lara Kuntz Group: iGEM12_TU_Munich (2012-09-25)
(+)-limonene synthase 1 with yeast consensus sequence for improved expression
This part is second version of BBa_K801060. It contains (+)-limonene synthase and the consensus sequence for enhanced expression in yeast. It does not contain a C-terminal Strep tag as BBa_K801060. The sequence does not contain a stop codon so that RFC 25 has to be used.
Does not contain stop codon. Needs to be used with RFC 25.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 107
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Categories
Parameters
None |