Part:BBa_K782079
10x[TALA] operator_CMV promoter_2xBFP:NLS
TALA labels represents TAL effector 1257 from zebrafish experiments (Sander et al., 2011).
Introduction
Our construct contain TALA binding sites that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.
Figure 1: Schematic representation of the construct.
Characterization
Results: Specific TAL binding sites were further characterized with other reporter constructs.
BFP was obtained from Evrogen.
References
Sander, J. D., Cade, L., Khayter, C., Reyon, D., Peterson, R. T., Joung, J. K., and Yeh, J.-R. J. (2011) Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nature Biotechnology 29, 697–698
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 720
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 507
Illegal AgeI site found at 12
Illegal AgeI site found at 347
Illegal AgeI site found at 372
Illegal AgeI site found at 707 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1396
Illegal BsaI.rc site found at 2052
Illegal BsaI.rc site found at 2757
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