Reporter

Part:BBa_K823028:Design

Designed by: Jara Radeck   Group: iGEM12_LMU-Munich   (2012-08-30)
Revision as of 10:15, 26 September 2012 by Jara (Talk | contribs) (→‎References)

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luc luciferase + RBS, codon optimized for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 625
    Illegal SapI site found at 853


Design Notes

codon optimized for Bacillus subtilis

prefix: (next nucleotide is then the a from atg); Includes RBS with correct spacing

GAGCAGAATTCGCGGCCGCTTCTAGAGTAAGGAGGAACTACT

suffix: (directly after the TAA)


TACTAGTAGCGGCCGCTGCAGGCTGA

Source

synthesis by gene art

References

Reference:[http://www.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/bioluminescent-reporters/ Promega]