Generator

Part:BBa_K819008

Designed by: ZHANG Zidong   Group: iGEM12_Peking   (2012-09-03)
Revision as of 10:03, 26 September 2012 by Zhangzidong (Talk | contribs)

Lux Operon under T7 promoter

Lux operon genes (from BBa_K325909) and related RBS are placed under T7 promoter. Cells transformed with this part can produce blue luminescence while no exogenous substrate is needed.

luxbrick under T7 promoter is very modular, because it is transcribed by T7 polymerase, which can be placed under any other promoter, forming a interface between luxbrick and other systems. Also, when transformed into BL21 cells, it can be induced with IPTG to reach a high expression level.


Usage and Biology

BL21 cells harboring T7-lux operon induced with IPTG at 5*10-5M is shown in the photo
The best temperature for cells to glow is 30oC rather than 37oC.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2012
    Illegal XhoI site found at 2842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4401
    Illegal BsaI.rc site found at 1410
    Illegal SapI.rc site found at 4726


[edit]
Categories
//cds/biosynthesis
//chassis/prokaryote/ecoli
//function/reporter/light
//plasmid/expression
Parameters
emission485nm
n/aLux Operon under T7 promoter
originVibrio.fischeri