Part:BBa_K786002

Sensory rhodopsin II (SRII) with HtrII & Tar, sensitive to blue-green light

Sensory Rhodopsin II have been fused with HtrII with the a linker peptide, only the membrane-proximal cytoplasmic domain have been kept, while the cytoplasmic domains have been replaced by that of Tar. Once the fusion protein was triggered, the histidine kinase (CheA) will be down regulated, leading to longer running period(Positive phototaxis), sensitive to blue-green light, with sensing spectra covering from 400-500 nm. We used it for sensing blur-green light to achieve phototaxis and switch for downstream gene expression.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 37
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 37
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 785
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 37
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 37
  Illegal NgoMIV site found at 140
  Illegal NgoMIV site found at 398
  Illegal AgeI site found at 1703
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1139
  Illegal SapI site found at 913