Plasmid_Backbone
pSBBs2E
Part:BBa_K823027:Design
Designed by: Jara Radeck Group: iGEM12_LMU-Munich (2012-08-21)
pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 91
Illegal NotI site found at 1622 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NgoMIV site found at 2591
Illegal NgoMIV site found at 4919 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 4743
Illegal SapI site found at 3660
Design Notes
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The clonging site also includes a rfp for selection in E.coli.
Source
This part is an igem compatible version of the Bacillus subtilis vector pAX01
References
[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]