Part:BBa_K769019
Promoter(high)-lumP-DT
BBa_K769011+Promoter(high)-lumP-DT
lumazine protein
Bacterial luciferase derived from Vibrio fischeri, which is a strain of luminous bacteria, shows brue-green light emission (wavelength is 495 nm). however, in luminous bacteria, there are strains that emit light with shorter wavelength than normal Vibrio fischeri (475nm,blue), such as Photobacterium phosphoreum and Photobacterium leiognathi. It is known that blue shift of these luminous bacterium is caused by lumazine protein (LumP). LumP shifts the wavelength by interacting with the luciferase. As a chromophore, it has 6,7-dimethyl-8-(1'-D-ribityl)lumazine (DMRL), and its size is about 21kDa.
Learning from previous reports, we decided to use these proteins for changing the color.
Improve the existing BioBrick Part
We used BioBrick part(BBa_K216007) coding lumazine protein. We thought that gene coding protein only is difficult to use for constructing device, so we added promoter and double terminator for this part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 397
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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