Generator
LacIM
Part:BBa_K819012
Designed by: ZHANG Hong Group: iGEM12_Peking (2012-09-03)
Revision as of 04:38, 26 September 2012 by Zhangzidong (Talk | contribs)
Luminesensor repressible LacIM generator
LacI mutant protein is placed under SulA408 promoter
SulA408 promoter (BBa_K819017) can be repressed by LexA408 protein or protein with LexA408 domain (e.g. the optimized luminesensor constructed by Peking 2012 iGEM), without disturbance from endogenous LexA protein in E.coli.
LacI mutant from BBa_K395400 can repress lac promoter. So this part can work as a connection between two systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/transcriptionalregulator/repressor
//chassis/prokaryote/ecoli
//function/cellsignalling
//chassis/prokaryote/ecoli
//function/cellsignalling
n/a | Luminesensor repressible LacIM generator |