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Composite
sboA-AmilG

Part:BBa_K818600

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-19)
Revision as of 23:17, 25 September 2012 by RenskevR (Talk | contribs) (→‎Usage and Biology)

AmilGFP with sboA regulator

This is a coding construct for B. subtilis to produce AmilGFP pigment in response to rotten meat volatiles in the environment. AmilGFP is a yellow pigment designed by iGEM Uppsala 2011 and the regulator, sboA, is designed by iGEM Groningen 2012.

Usage and Biology

Expression in E. coli

SboA-AmilGFP is strongly expressed in E. coli, on plate in liquid culture, at normal growth conditions. On plate, the construct is expressed less strongly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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