Measurement

Part:BBa_K911001:Experience

Designed by: Oliver Meacock   Group: iGEM12_Cambridge   (2012-09-07)
Revision as of 22:31, 25 September 2012 by Olijme (Talk | contribs) (→‎Applications of BBa_K911001)

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Applications of BBa_K911001

BBa_K911001 has been inserted into the following construct:

MgConstruct1.jpg

The sequence of this construct has been verified.

In order to test it, we transformed the construct into e.coli and attempted to induce GFP expression with both increasing magnesium concentrations and increasing IPTG concentrations. The cells were then interrogated at the correct GFP absorbtion and emission wavelengths (450nm and 545nm respectively) in a plate reader.

The results of this assay are shown below.

MgEcoliAssay.png

As can be seen, the assay did not give the expected results. At differing IPTG concentrations, the response to magnesium seems to have inverted.

The construct was then transformed into Bacillus subtilis, with a view to repeat the same assay in this new chassis. Because the part originally comes from bacillus, we hoped that this might give more useful data.

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