Measurement

Part:BBa_K911003:Design

Designed by: Oliver Meacock   Group: iGEM12_Cambridge   (2012-09-12)
Revision as of 22:07, 25 September 2012 by Olijme (Talk | contribs) (→‎Design Notes)

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Fluoride Sensitive Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 26
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because this is a positive regulator (it ceases transcriptional termination in the presence of F- ions), it was decided that it should be placed upstream of a direct reporter. In this case, lacZ was used as our reporter. We have included the lysine promoter as part of this biobrick as a reliably constitutively active promoter, ensuring that the rate of transcription of downstream genes should only be affected by the rate of transcriptional attenuation by the fluoride riboswitch.

Source

  • Bacillus Cereus Genome
  • Many thanks to the Breaker lab at Yale University, who provided us with the original lacZ reporter construct, as well as a knockout strain of bacillus subtilis.

References

  • Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science (2012) vol. 335 pp. 233 - 235.