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Coding

Part:BBa_K808027

Designed by: Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Philipp Rottmann, Rene Sahm, Arne Wehling   Group: iGEM12_TU_Darmstadt   (2012-09-02)
Revision as of 21:47, 25 September 2012 by Arne (Talk | contribs)

EstA : inactive fusion protein for bacterial surface expression

EstA is a membrane bound but inactive esterase from Pseudomonas aeruginosa. It can be used (unlike https://parts.igem.org/Part:BBa_K633001 BBa_K633001) for the cell surface expression of enzymes. Similiar to OmpA it allows to perform bacterial display of e.g. hydrolases like it has already been shown by Becker, Theile, Heppeler & Michalczyk, 2005. We used EstA to create our chimeric protein BBa_K808030(Figure 1).

Figure 1:BBa_K808030 EstA (red & grey) anchoring a second protein (pNB-Est13 in yellow) into a biological membrane


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 97
    Illegal NgoMIV site found at 235
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1144
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/membrane
//cds/membrane/extracellular
Parameters
protein