Reporter

Part:BBa_K782077

Designed by: Fedja Pavlovec   Group: iGEM12_Slovenia   (2012-09-24)
Revision as of 21:40, 25 September 2012 by UrosZupancic (Talk | contribs)

12x[NicTAL] operator_CMV promoter_BFP

Introduction

Our construct contain twelve binding sites for NicTAL12 (sequence) that are cloned upstream of CMV promoter. Downstream of CMV is blue fluorescent protein. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Nicbfp.png

Figure 1: Schematic representation of the construct.


Characterization

Results:

BFP was obtained from Evrogen.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 16
    Illegal AgeI site found at 248
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1140
    Illegal BsaI.rc site found at 1796


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Parameters
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