Reporter

Part:BBa_K782080

Designed by: Fedja Pavlovec   Group: iGEM12_Slovenia   (2012-09-24)
Revision as of 21:27, 25 September 2012 by UrosZupancic (Talk | contribs)

10x[TALB] operator_CMV promoter_2xBFP:NLS

TALB labels represents TAL effector 1297 from zebrafish experiments (Sander et al., 2011).

Introduction

Our construct contain TALB binding sites that are cloned upstream of CMV promoter. Downstream of CMV is a double blue fluorescent protein with NLS domain, causing protein to migrate into the nucleus. BFP is a monomeric fluorescent protein with excitation maximum at 402 nm and emision maximum at 457 nm.

Bbfp2.png

Figure 1: Schematic representation of the construct.


Characterization

Results:

BFP was obtained from Evrogen.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 147
    Illegal NgoMIV site found at 507
    Illegal AgeI site found at 12
    Illegal AgeI site found at 347
    Illegal AgeI site found at 372
    Illegal AgeI site found at 707
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1396
    Illegal BsaI.rc site found at 2052
    Illegal BsaI.rc site found at 2757


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