Deleted
Experience: None
Not Used
Get This Part
Reporter

Part:BBa_K883702

Designed by: Lisa Sonnenberg   Group: iGEM12_Wageningen_UR   (2012-09-24)
Revision as of 19:43, 25 September 2012 by Lisamsonne (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Figure 1: cross-section view of a E-/ K-coil heterodimer'

IPTG induced promoter + GFP fused to a coiled coil

GFP with the E-coil at the N-terminus behind an IPTG induced promoter to use it as reporter for the E- and K-coil [http://2012.igem.org/Team:Wageningen_UR/Coil_system Plug and Apply system]

Usage and Biology

The E-coil present in this reporter protein is one part of the Plug and Apply (PnA) system consisting of a combination of E- and K-coil. These coils consist of α-helical repeats and have a strong affinity to each other due to electrostatic interactions (with K-coil positively charged and E-coil negatively charged).

Using this system one can bind two proteins noncovalently but specifically to each other.

This brick can be used for confirmation of the Plug and Apply system using E- and K-coils as well as for detection of proteins that can be fused to the K-coil.

Figure 2: protein folding prediction of the E-coil attached to a GFP-molecule'


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 930


[edit]
Categories
Parameters
None