Composite

Part:BBa_K741019:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-08-29)
Revision as of 18:48, 25 September 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

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pRM-anticro-T-pRM-RBS-lysis-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If a lambda phage infects a bacteria, it will express protein CI and Cro. The CI will activate the promoter pRM and the anticro and the lysis(for E7 colicin) will be produced. The anticro is the antisense RNA of cro and can repress the expression of cro. The lysis can destroy the inner membrane and activate the OMPLA(Outer Membrane Phospholipase, an enzyme in many Gram-negative bacteria) to destroy the outer membrane. Then the host infected and the lambda phage will die.

Source

BBa_K741016 BBa_K741014

References