Composite

Part:BBa_K741011:Design

Designed by: Wuyang Chen   Group: iGEM12_USTC-China   (2012-07-24)
Revision as of 18:36, 25 September 2012 by MelodyChen (Talk | contribs) (→‎Design Notes)

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plac-anticro-T-pCon(0.244)-RBS-crogfp-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
    Illegal BglII site found at 1481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918


Design Notes

The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.

Source

BBa_K741008 BBa_K741010

References