Composite

Part:BBa_K934022

Designed by: Toshiki Hashimoto   Group: iGEM12_Tokyo_Tech   (2012-09-16)
Revision as of 17:48, 25 September 2012 by Toshiki (Talk | contribs)

Plux-LasI

We constructed this part by combining R0062 and K081016. This part starts generating LasI enzyme in the presence of LuxR-3OC6HSL complex.

Plux-LasI result.png

In the presence of 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, “Las reporter cell” was activated and GFP was expressed. Thus, the expression of GFP in “Las reporter cell” is dually regulated by 3OC12HSL produced by “3OC6HSL dependent 3OC12HSL producer cell”, this result shows that Plux-lasI(K934022) synthesized 3OC12HSL.

For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work] in Tokyo_Tech 2012 wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None