Coding

Part:BBa_K896006

Designed by: Shih-Hao Chen   Group: iGEM12_NYMU-Taipei   (2012-09-22)
Revision as of 17:34, 25 September 2012 by Adonis (Talk | contribs)

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NirN-NirS (Nitrite->Nitric oxide reductase)

Cloning of NirN+NirS gene:

Nitrite reductase is composed of several different subunits. Together, it can reduce nitrite to nitric oxide. We clone all these subunits together on the same plasmid in order to grant cyanobacteria a functional protein.

NirN+S cloning.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1703
    Illegal BamHI site found at 1483
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 168
    Illegal NgoMIV site found at 220
    Illegal NgoMIV site found at 604
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 2305
    Illegal AgeI site found at 3169
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2347
    Illegal BsaI.rc site found at 2479


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Parameters
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