Regulatory

Part:BBa_K875002:Experience

Designed by: Federico Colombo, Elisa Clagnan   Group: iGEM12_Trieste   (2012-09-23)
Revision as of 17:20, 25 September 2012 by Colombofederico (Talk | contribs) (→‎Applications of BBa_K875002)

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Applications of BBa_K875002

Trieste Team 2012 used this inducible promotor in the first phase of their project to test every construct that could have been toxic for the host strain, if expressed in a constitutive way.

How it works:

The host strain contains the low copy plasmid pREP4 which constitutively expresses the Lac repressor protein encoded by the lacI gene. The lac repressor binds the operator sequence preventing the transcription. The Lac repressor protein is rapidly inactivated by the binding of isopropyl-β-thiogalactoside (IPTG)

User Reviews

UNIQfdd57f4a2071f250-partinfo-00000000-QINU UNIQfdd57f4a2071f250-partinfo-00000001-QINU