Part:BBa_K750111

PtetGLT:GFP(lva) expression system controlled by (anhydro)tetracyclin

Description

This part includes TetR composition, promoter Pter, RBS of 1.0 strength, a unstable GFP reporter and the double terminator. It is designed to work when (anhydro)tetracyclin is added. In the digital display part of our project E.Lumoli, we need different induction to activate the circuit and make GFP be produced or be degraded. The inspiration of this part comes from BBa_K145280(designed by iGEM08_KULeuven). We planed to use this biobrick in our project at the very start, but when we test this device by using 140 ng/ul atc(protocol from team KULeuven), the device could not work. So we changed the RBS from 0.3 strength to 1.0 strength. This time, the device worked.

performance

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1615