Generator

Part:BBa_K792009

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-25)
Revision as of 06:05, 25 September 2012 by Manugimenez (Talk | contribs)

Yeast exportable His-rich peptide w/enhanced import (1)

This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide).

The high level structure of the device is:

  • Kozak consensus sequence for initiation of translation
  • Signal peptide that targets the product of the gene for secretion
  • Trojan peptide, to increase internalization in target cell
  • Payload: this is the exported amino acid rich domain of the protein

Note: this part has been submitted to the registry using a direct synthesis dna sample, so it does not contain scars from BB assembly standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None