Regulatory
CP44

Part:BBa_K864511

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 05:58, 25 September 2012 by Agynna (Talk | contribs)

CP44 constitutive promoter

This is a constitutive promoter characterized in E. coli and Lactococcus lactis. It has a strength of 34 (in E coli) and 12 (in L lactis) Miller units in the β-Galactosidase assay of Jensen & Hammer (1998). That makes it into a quite strong promoter in E coli and a rather weak promoter in L lactis (note that the scales are cannot be directly compared).

Usage and Biology

The CP series of Jensen & Hammer are useful as constitutive promoters when the restriction sites included in the Anderson promoter collection are illegal.

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC124675/] P. Jensen, K Hammer Appl: The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters Environ Microbiol. 64.1 (1998) 82–87.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None