Part:BBa_K819000:Design

Luminescence-responsive Promoter+GFP(BBa_E0840)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.

Source

ColE7 promoter, ColE plasmid