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Part:BBa_K819000:Design

Designed by: Haoqian Zhang   Group: iGEM12_Peking   (2012-08-31)
Revision as of 04:48, 25 September 2012 by Zhanghong (Talk | contribs)

Luminescence-responsive Promoter+GFP(BBa_E0840)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The LexA binding site was mutated to guarantee that the endogenous LexA will not disturb the function of this promoter. This mutated LexA binding site within luminescence-responsive promoter can be recognized by corresponding mutated LexA DNA binding domain.


Source

ColE7 promoter, ColE plasmid

References