Regulatory

Part:BBa_K911008:Design

Designed by: Stuart Bell   Group: iGEM12_Cambridge   (2012-09-24)
Revision as of 00:57, 25 September 2012 by Scb67 (Talk | contribs) (→‎References)

Fast Germination (B.subtilis)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of this BioBrick is based on a plasmid (pPS3393)from Wang et al. (2011)

Wang, G., Yi, X., Li, Y.-Q., Setlow, P. Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination(2011) J. Bacteriology, 193 (9), pp. 2301-2311.


Source

spoVAA and PsspB from B.subtilis Genome.

References

Wang, G., Yi, X., Li, Y.-Q., Setlow, P. Germination of individual Bacillus subtilis spores with alterations in the GerD and SpoVA proteins, which are important in spore germination(2011) J. Bacteriology, 193 (9), pp. 2301-2311.