Reporter
redq

Part:BBa_K864502:Design

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 00:41, 25 September 2012 by Agynna (Talk | contribs) (→‎Design Notes)

RFP Coding Device, constitutive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The CP44 promoter was chosen to be similar in strength to the LacI promoter, and to be used as a replacement for that one in the standard RFP cassette.

This part has not been assembled as a stand-alone BioBrick. It is a logical part of the pMB1ori-redq BBa_K864101, to be used as a default insert in low and middle copy backbones.

Source

A BBa_J04450 RFP reporter, but modified by PCR mutagenesis.

References