Regulatory

Part:BBa_K911008

Designed by: Stuart Bell   Group: iGEM12_Cambridge   (2012-09-24)
Revision as of 00:12, 25 September 2012 by Registry (Talk | contribs)

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Fast Germination (B.subtilis)

This part upregulates an operon responsible for germination rate. Bacillus subtilis spores germinate in response L-Alanine. Up-regulation of the spoVA operon increases germination rate in response to L-Alanine. The promoter for the B.subtilis sspB gene (PsspB) is more active than the endogenous spoVA promoter. It is also active during germination. Expression of the spoVA operon under PsspB increases the germination rate.

This construct consists mainly of the sspB promoter followed by the first 354bp of the spoVA operon (first 354bp of the spoVAA gene). Since B.subtilis exhibits accurate and efficient homologous recombination, a single cross-over event between the spoVAA region in the BioBrick and the endogenous spoVAA sequence inserts the sspB promoter upstream of the spoVA operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None