Generator
D0

Part:BBa_K738000:Design

Designed by: Huachun Liu   Group: iGEM12_ZJU-China   (2012-07-13)
Revision as of 19:14, 24 September 2012 by Smile (Talk | contribs) (→‎Source)

RNA Scaffold generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Generally, RNA strands have a half-life that ranges between 40 seconds and 60 minutes. We use E.coli strain BL21*(DE3), which possess a mutation in the RNAaseE (rne131) gene to limit RNA degradation.

Source

Dr. Camille J. Delebecque offer us d0 secquence in plsmid backbone pETDuet-1(Novagan).

References

C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, “Organization of intracellular reactions with rationally designed RNA assemblies” Science 333, 470-474 (2011).