Composite

Part:BBa_K917010

Designed by: Elitsa Peeva   Group: iGEM12_Edinburgh   (2012-09-24)
Revision as of 17:10, 24 September 2012 by Registry (Talk | contribs)

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Kanamycin resistance+lac promoter+RFP+sacB selection-counterselection cassette

The Red Fluorescent Protein (RFP) and SacB, a Bacillus subtilis levansucrase created by Edinburgh team 2010 have been placed under the control of the lac promoter in addition to kanamysin resitance gene. Function is to creaye a selection-counterselection cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1756
    Illegal AgeI site found at 1868
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None