Device

Part:BBa_K883001

Designed by: Jeroen Bosman   Group: iGEM12_Wageningen_UR   (2012-09-23)
Revision as of 16:14, 24 September 2012 by Jeroenbosman (Talk | contribs) (→‎Usage and Biology)

CCMV wt coat protein under IPTG induced promoter

CCMV is an abriviation of the Cowpea Chlorotic Mottle Virus, one of the most studied viruses in producing Virus Like Particles (VLPs). This part is the coding sequence of the coat protein of the CCMV virus. When expressed in Escherichia coli, these coat proteins can be harvested and be assembled in vitro using the Wageningen_UR iGEM 2012 protocols (http://2012.igem.org/Team:Wageningen_UR/Protocol/StartupCCMV) . This part is provided for the iGEM community so that they can use this part to produce these VLPs for their one own use, including but not limited to packaging, making fusion proteins to the N/C terminal. This all for to be used in a wide variety of applications. for ideas what kind of applications can be mediated with CCMV VLPs, check http://2012.igem.org/Team:Wageningen_UR/Applications



Usage and Biology

Protocols for production and purification:

  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/StartupCCMV| CCMV production protocol part 1]
  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/DialysisCCMV| CCMV production protocol part 2]
  • [http://2012.igem.org/Team:Wageningen_UR/Protocol/RoundupCCMV| CCMV purification protocol]


This part is made from the wild type CCMV coat protein. CCMV is the abbreviation of Cowpea Chlorotic Mottle Virus, and is a well studied black eyed pea infecting virus. It is a virus in the family Bromoviridae, and it is one of the most studied plant viruses. As from the discovery of the virus in 1967, it was an interesting virus due to the easy cultivation and harvesting procedures involved. Interest in this virus is especially in the ability to disassemble the virus, remove the content and refold the empty particle simply by altering the pH. This makes this virus one of the first and best studied Virus Like Particles. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 368
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

[edit]
Categories
//viral_vectors
Parameters
None