Composite

Part:BBa_K929004

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)
Revision as of 14:43, 24 September 2012 by ChristopherKressler (Talk | contribs)

modified AID+eGFP

This is a composite part from the existing parts: modified_AID (BBa_K929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see [https://parts.igem.org/Part:BBa_K929004:Design Part_Design. This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 1225
    Illegal SapI site found at 209


[edit]
Categories
Parameters
None