Regulatory

Part:BBa_K733002

Designed by: WANG, Yuqi   Group: iGEM12_HKUST-Hong_Kong   (2012-09-16)
Revision as of 12:03, 24 September 2012 by Feisun0718 (Talk | contribs)

xylR+PxylA: A xylose inducible promoter with its transcriptional regulator.


The xylose inducible promoter used to construct this part is amplified from the integration plasmid pAX01. （Zeigler 2002）This plasmid contains a xylose inducible promoter which is originated from Bacillis megaterium. The xylose inducible promoter we constructed contains promoter pxylA which located within xylose operon, originally to drive the expression of xylA (xylose isomerase coding gene) and xylB (xylulose kinase). xylR with its promoter located at upstream of xylose operon is also included in our parts. It encodes xyl repressor which binds to xyl operator in the absence of xylose, repressing transcription event. In the presence of glucose, glucose-6-phosphate metabolized from glucose can compete with xylose in the binding site of xylose on XylR. In addition, glucose itself is also supposed to be a low efficiency inducer for XylR. (DAHL, 1997) Therefore while xylose induces the transcription, the existence of glucose, to some extent, represses gene transcription.


Pxyl1.png Pxyl2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Reference

Zeigler, D. (2002). Integration Vectors for Gram-Positive Bacteria (7 ed.). Columbus: The Bacillus Genetic Stock Center.

Dahl, M. K., D. Schmiedel, and W. Hillen. 1995. Glucose and glucose-6-phosphate interaction with Xyl repressor proteins from Bacillus spp. May contribute to regulation of xylose utilization. J. Bacteriol. 177:5467–5472.

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