Other

Part:BBa_K802005:Design

Designed by: Sandu Ioana   Group: iGEM12_Lyon-INSA   (2012-09-21)
Revision as of 00:25, 24 September 2012 by Pishki (Talk | contribs) (→‎Design Notes)

iGEM linker for shuttle vectors BBa_K802003 and BBa_K802004


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker was especially designed to contain the iGem restriction sites in the right order. It was obtained by the hybridization of two primers:
Forward: (5’)aattcgcggccgcttctagagaatactagtagcggccgctgcaga(3’)
Reverse: (5’)agcttctgcagcggccgctactagtattctctagaagcggccgcg(3’)

The percentage of the hybridization of the two primers is 91%. The only difference between the two consists in 8 nucleotides: 4 at the 5’end in the case of the Forward primer (which represents the sticky end compatible with a digested HindIII site) and the other 4 at 3’end in the case of the Reverse primer (which represents the sticky end compatible with a digested EcoRI site).

Source

The linker was obtained by the hybridization of two synthesized primers.

References