Regulatory
Part:BBa_K799009:Design
Designed by: Jiarui Wang Group: iGEM12_Johns_Hopkins-Wetware (2012-09-23)
Ethanol/Stress Inducible YMR251W-A Promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 120
Illegal PstI site found at 277 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 169
Illegal NheI site found at 355
Illegal PstI site found at 277 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 120
Illegal PstI site found at 277 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 120
Illegal PstI site found at 277 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Golden Gate(RFC#88) compatible
Source
Amplified from the yeast strain S288C