Coding

Part:BBa_K909008

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)
Revision as of 20:06, 23 September 2012 by Registry (Talk | contribs)

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tetR-DBD-UVR8 fusion protein

Tetracycline repressor DNA binding domain fusion with truncated version of UVR8 (tetR-DBD-UVR8). UVR8 is a UV-B sensing receptor in plants necessary for UV-B protection. In dark state UVR8 forms a dimer which is monomerized by the UV-B light (280-315 nm). A truncated version of UVR8 (14-440 amino acids) was fused with tetR DNA binding domain (tetR-DBD), lacking of dimerization domain. Such mutant shows no repression of Ptet promoter, however fusion with dimerizing protein (UVR8) returns tetR-DBD activity. Later UV-B exposure breaks UVR8 dimer and releases tetR-DBD-UVR8 from Ptet, activating transcription. Thus, this tetR-DBD-UVR8 can be used as a one step UV-B on switch.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 418
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 382
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 902
    Illegal PstI site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 433


[edit]
Categories
//cds/membrane/receptor
Parameters
None